1 WSOC table

1.1 soils

mg/g soil

Treatment CPCRW DWP SR
Drought 0.85 ± 0.2 a 0.72 ± 0.37 a 0.6 ± 0.04 a
Field Moist 0.69 ± 0.07 a 0.35 ± 0.05 a 0.61 ± 0.05 a
Saturated 0.59 ± 0.05 a 0.39 ± 0.06 a 0.55 ± 0.06 a
Time Zero 0.78 ± 0.1 a 0.51 ± 0.08 a 0.43 ± 0.04 a

1.2 pores

mg/L

Treatment 1.5 kPa CPCRW 1.5 kPa DWP 1.5 kPa SR 50 kPa CPCRW 50 kPa DWP 50 kPa SR
Drought 111.82 ± 41.45 a 69.02 ± 13 a 13.9 ± 2.43 ab 186.86 ± 62.65 a 69.97 ± 13.39 a 19.3 ± 8.32 ab
Field Moist 21.22 ± 3.73 b 46.33 ± 11.94 a 18.68 ± 2.36 a 41.17 ± 12.67 ab 52.43 ± 14.74 a 19.82 ± 2.93 ab
Saturated 52.82 ± 13.98 ab 42.08 ± 7.34 a 23.16 ± 3.29 a 63.4 ± 20.84 ab 82.7 ± 16.52 a 24.07 ± 2.43 a
Time Zero 26.3 ± 2.78 ab 76.68 ± 21.76 a 6.03 ± 0.57 b 29.14 ± 4.41 b 92.33 ± 22.22 a 7.28 ± 0.34 b

1.3 soils, pores combined

2 FTICR Pores – figures

2.1 native SOM

Van Krevelen plot for TZSat pores

(#fig:vk_baseline)Van Krevelen plot for TZSat pores

2.2 treatment effect – all peaks

Van Krevelen plot for treatments

(#fig:vk_treatments)Van Krevelen plot for treatments

2.3 treatment effect – unique peaks

Van Krevelen plot for uniqiue peaks

(#fig:vk_unique)Van Krevelen plot for uniqiue peaks

2.4 treatment effect – relative abundance

Relative abundance

(#fig:rel_abund)Relative abundance

2.5 treatment effect – NOSC

NOSC for CPCRW

(#fig:NOSC_c)NOSC for CPCRW

NOSC for DWP

(#fig:NOSC_d)NOSC for DWP

NOSC for SR

(#fig:NOSC_s)NOSC for SR

2.6 aromatic peaks

aromatic peaks

Figure 2.1: aromatic peaks

3 FTICR Pores – tables

3.1 peaks

Class CPCRW drought CPCRW field moist CPCRW saturation CPCRW time zero saturation DWP drought DWP field moist DWP saturation DWP time zero saturation SR drought SR field moist SR saturation SR time zero saturation
AminoSugar 912 27 101 95 156 78 118 1064 19 138 235 125
Carb 1220 39 186 92 305 24 120 1144 23 75 43 21
ConHC 582 408 558 967 372 396 815 529 11 55 59 210
Lignin 8749 3999 6125 7846 5015 3624 5602 5716 2268 3741 3071 3524
Lipid 813 132 713 574 586 195 447 606 471 891 999 520
Other 64 27 7 35 44 6 12 84 7 18 13 12
Protein 2831 255 1523 890 1796 606 1094 2474 814 1115 2143 1137
Tannin 993 285 452 577 289 318 619 751 13 40 33 110
total 16180 5199 9676 11117 8584 5256 8865 12435 3642 6156 6657 5728
UnsatHC 16 27 11 41 21 9 38 67 16 83 61 69
Class CPCRW drought CPCRW field moist CPCRW saturation CPCRW time zero saturation DWP drought DWP field moist DWP saturation DWP time zero saturation SR drought SR field moist SR saturation SR time zero saturation
AminoSugar 508 72 122 162 117 93 19 958 65 205 57 172
Carb 792 89 123 74 380 40 47 1219 14 96 34 37
ConHC 195 556 353 565 125 500 609 196 86 49 82 113
Lignin 4393 5504 5737 5642 3827 4852 3447 3414 2279 2722 1982 1967
Lipid 472 588 1081 707 548 465 358 555 845 817 498 608
Other 110 28 40 23 58 24 11 106 11 17 NA 18
Protein 1802 1308 2130 1957 1540 979 605 2259 1081 1802 433 1584
Tannin 385 372 256 389 61 372 165 490 33 35 31 39
total 8670 8577 9904 9588 6677 7358 5294 9257 4469 5799 3203 4611
UnsatHC 13 60 62 69 21 33 33 60 55 56 86 73

3.2 unique peaks

Class CPCRW drought CPCRW field_moist CPCRW saturation DWP drought DWP field_moist DWP saturation SR drought SR field_moist SR saturation
AminoSugar 217 NA 2 5 2 3 NA 11 23
Carb 240 1 NA 8 NA NA 2 10 1
ConHC 6 4 4 6 5 57 NA NA 4
Lignin 328 12 38 45 14 67 2 26 91
Lipid 26 1 28 31 NA 12 30 18 70
Other 10 NA NA 5 NA NA NA 2 2
Protein 282 NA 24 93 7 6 11 17 175
Tannin 104 NA 5 1 2 32 NA NA 3
UnsatHC NA 2 NA NA NA 2 NA 3 2
total 1213 20 101 194 30 179 45 87 371
Class CPCRW drought CPCRW field_moist CPCRW saturation DWP drought DWP field_moist DWP saturation SR drought SR field_moist SR saturation
AminoSugar 118 NA 3 7 7 NA NA 14 3
Carb 177 4 3 6 3 NA NA 16 1
ConHC 6 3 3 4 29 27 8 10 8
Lignin 71 102 107 23 230 2 59 96 19
Lipid 8 5 88 25 16 4 63 22 9
Other 19 NA NA 6 4 NA 1 4 NA
Protein 86 16 61 49 15 NA 25 81 9
Tannin 15 6 NA 1 34 NA 4 5 1
UnsatHC NA 7 3 NA 5 3 NA NA 6
total 500 143 268 121 343 36 160 248 56

3.3 relative abundance

group CPCRW drought CPCRW field moist CPCRW saturation CPCRW time zero saturation DWP drought DWP field moist DWP saturation DWP time zero saturation SR drought SR field moist SR saturation SR time zero saturation
AminoSugar 4.35 ± 0.66 a 0.2 ± 0 b 0.53 ± 0.06 b 0.37 ± 0.02 b 1.38 ± 0.38 b 0.79 ± 0.07 b 0.61 ± 0.12 b 8.79 ± 1.49 a 0.31 ± 0.09 a 1.24 ± 0.24 a 1.66 ± 0.34 a 2.18 ± 1.37 a
Carb 10.91 ± 2.17 a 0.31 ± 0.01 b 0.9 ± 0.25 b 0.42 ± 0.05 b 4.06 ± 1.1 b 0.33 ± 0.01 b 0.86 ± 0.15 b 9.91 ± 1.31 a 0.3 ± 0.05 a 1.03 ± 0.54 a 0.38 ± 0.03 a 0.17 ± 0.02 a
ConHC 1.37 ± 0.34 b 2.58 ± 0.51 ab 2.5 ± 0.45 ab 4.14 ± 1.04 a 2.35 ± 0.33 a 3.66 ± 0.85 a 4.96 ± 1.11 a 2.47 ± 0.26 a 0.42 ± 0.19 a 0.51 ± 0.15 a 1.25 ± 0.52 a 1.44 ± 0.32 a
Lignin 56.09 ± 2.88 c 82.96 ± 1.55 a 69.89 ± 1.46 b 75.73 ± 2.43 ab 55.56 ± 2.55 ab 71.02 ± 2.08 a 58.9 ± 9.08 a 33.19 ± 3.36 b 42.7 ± 8.42 a 36.06 ± 5.64 a 28.39 ± 4.35 a 40.32 ± 11.73 a
Lipid 7.07 ± 1.09 a 5.52 ± 2.18 a 9.61 ± 1.49 a 8.66 ± 1.76 a 11.53 ± 1.44 a 9.36 ± 1.09 a 18.88 ± 10.45 a 7.74 ± 2.81 a 36.81 ± 7.51 a 43.72 ± 6.42 a 38.67 ± 7 a 33.89 ± 9.83 a
Protein 16.11 ± 1.74 a 3.97 ± 0.08 b 12.8 ± 0.71 a 7 ± 0.8 b 22.26 ± 2 a 10.7 ± 1.54 b 10.55 ± 1.35 b 30.92 ± 3.15 a 17.24 ± 4.14 a 14.49 ± 2.04 a 27 ± 3.64 a 17.71 ± 5.26 a
Tannin 3.37 ± 0.46 a 3.93 ± 0.92 a 3.14 ± 0.6 a 2.88 ± 0.43 a 1.52 ± 0.21 b 3.16 ± 0.46 ab 3.95 ± 0.93 ab 5.18 ± 0.31 a 0.25 ± 0.15 a 0.26 ± 0.07 a 0.22 ± 0.05 a 1.54 ± 1.01 a
UnsatHC 0.1 ± 0.03 a 0.36 ± 0.04 a 0.59 ± 0.44 a 0.66 ± 0.32 a 0.76 ± 0.32 a 0.87 ± 0.32 a 1.18 ± 0.53 a 1.21 ± 0.33 a 1.81 ± 0.79 a 2.55 ± 0.35 a 2.33 ± 0.46 a 2.56 ± 0.89 a
Other 0.63 ± 0.17 a 0.16 ± 0.02 b 0.04 ± 0 b 0.13 ± 0.04 b 0.58 ± 0.08 a 0.11 ± 0.02 b 0.11 ± 0.03 b 0.59 ± 0.06 a 0.16 ± 0.05 a 0.13 ± 0.03 a 0.11 ± 0.04 a 0.2 ± 0.05 a
total = 100%
group CPCRW drought CPCRW field moist CPCRW saturation CPCRW time zero saturation DWP drought DWP field moist DWP saturation DWP time zero saturation SR drought SR field moist SR saturation SR time zero saturation
AminoSugar 4.3 ± 0.52 a 0.33 ± 0.05 b 0.54 ± 0.1 b 0.82 ± 0.12 b 1.03 ± 0.16 b 0.66 ± 0.11 b 0.28 ± 0.1 b 7.76 ± 2.46 a 0.66 ± 0.23 a 1.63 ± 0.5 a 0.59 ± 0.12 a 1.84 ± 0.3 a
Carb 13.15 ± 2.28 a 0.4 ± 0.08 b 0.46 ± 0.05 b 0.36 ± 0.05 b 8.32 ± 1.22 ab 0.32 ± 0.03 b 0.8 ± 0.24 b 10.99 ± 3.48 a 0.13 ± 0.02 b 1.61 ± 0.61 a 0.79 ± 0.3 ab 0.51 ± 0.08 ab
ConHC 1.02 ± 0.23 a 2.26 ± 0.21 a 1.36 ± 0.42 a 2.55 ± 0.68 a 2.3 ± 0.29 b 3.1 ± 0.91 b 6.98 ± 1.16 a 1.66 ± 0.54 b 1.52 ± 0.45 a 0.71 ± 0.34 a 0.58 ± 0.12 a 1.29 ± 0.31 a
Lignin 47.39 ± 6.34 a 58.67 ± 9.42 a 52.55 ± 3.27 a 49.34 ± 5.81 a 43.38 ± 4.12 a 56.46 ± 1.95 a 54.11 ± 7.25 a 15.26 ± 3.77 b 30.2 ± 2.11 a 28.94 ± 10.2 a 19.26 ± 2.9 a 15.94 ± 0.84 a
Lipid 12.44 ± 5.18 a 24.17 ± 8.26 a 24.29 ± 2.66 a 24.23 ± 4.1 a 21.63 ± 3.13 a 20.84 ± 1.54 a 21.17 ± 5.37 a 29.39 ± 12.34 a 43.37 ± 1.9 a 44.84 ± 11.52 a 65.05 ± 3.58 a 43.82 ± 6.28 a
Protein 16.57 ± 1.96 a 11.57 ± 1.44 a 18.95 ± 2.03 a 19.93 ± 2.86 a 20.41 ± 2.14 b 14.44 ± 1.13 b 12.93 ± 2.3 b 30.07 ± 2.2 a 18.58 ± 1.49 ab 20.2 ± 4.83 ab 9.49 ± 0.92 b 33.15 ± 5.46 a
Tannin 2.28 ± 0.57 a 1.85 ± 0.21 a 1.04 ± 0.21 a 1.75 ± 0.27 a 0.44 ± 0.1 b 2.46 ± 0.25 ab 1.92 ± 0.51 ab 3.15 ± 1.06 a 0.22 ± 0.1 a 0.42 ± 0.11 a 0.18 ± 0.06 a 0.21 ± 0.05 a
UnsatHC 0.15 ± 0.05 b 0.65 ± 0.09 ab 0.68 ± 0.18 ab 0.96 ± 0.21 a 1.57 ± 1.11 a 1.53 ± 0.36 a 1.66 ± 0.26 a 0.97 ± 0.15 a 5.05 ± 1.66 a 1.56 ± 0.21 a 4.06 ± 0.85 a 2.9 ± 0.81 a
Other 2.71 ± 1.85 a 0.1 ± 0.03 a 0.13 ± 0.02 a 0.07 ± 0.02 a 0.9 ± 0.12 a 0.19 ± 0.04 bc 0.17 ± 0.03 c 0.75 ± 0.24 ab 0.26 ± 0.1 a 0.11 ± 0.02 a 0 ± 0 a 0.35 ± 0.15 a
total = 100%

4 FTICR Soil – figures

4.1 native SOM

Van Krevelen plot for TZSat pores

(#fig:soil_vk_baseline)Van Krevelen plot for TZSat pores

4.2 treatment effect – all peaks

Van Krevelen plot for treatments

(#fig:soil_vk_treatments)Van Krevelen plot for treatments

4.3 treatment effect – unique peaks

Van Krevelen plot for uniqiue peaks

(#fig:soil_vk_unique)Van Krevelen plot for uniqiue peaks

4.4 treatment effect – relative abundance

Relative abundance

(#fig:soiL_rel_abund)Relative abundance

4.5 NOSC

NOSC for CPCRW

(#fig:soil_NOSC_c)NOSC for CPCRW

4.6 aromatic peaks

aromatic peaks

(#fig:soil_aromatic)aromatic peaks

5 FTICR Soil – tables

5.1 relative abundance

group CPCRW baseline CPCRW drought CPCRW field moist CPCRW saturation CPCRW time zero saturation DWP baseline DWP drought DWP field moist DWP saturation DWP time zero saturation SR baseline SR drought SR field moist SR saturation SR time zero saturation
AminoSugar 2.69 ± 0.32 ab 1.48 ± 0.12 b 2.19 ± 0.48 ab 3.37 ± 0.34 a 2.94 ± 0.35 a 6.49 ± 0.45 a 1.82 ± 0.18 b 2.05 ± 0.13 b 1.74 ± 0.2 b 2.75 ± 0.24 b 2.82 ± 0.26 b 3.45 ± 0.32 ab 4.16 ± 0.16 a 3.37 ± 0.1 ab 3.16 ± 0.27 b
Carb 10.82 ± 1.88 ab 4.17 ± 0.92 b 9.6 ± 2.33 ab 10.78 ± 1.06 ab 13.53 ± 1.87 a 19.77 ± 0.65 a 6.38 ± 0.94 c 9.32 ± 0.53 c 9 ± 1.02 c 14.22 ± 1.66 b 11.72 ± 1.38 ab 15.55 ± 1.14 a 12.38 ± 0.83 ab 12.8 ± 0.78 ab 10.39 ± 0.75 b
ConHC 6.45 ± 0.92 a 8.29 ± 1.21 a 7.07 ± 0.6 a 6.01 ± 0.53 a 5.46 ± 0.63 a 7.27 ± 0.47 a 10.05 ± 0.47 a 9.14 ± 0.97 a 10.54 ± 1.27 a 9.77 ± 0.37 a 13.77 ± 0.89 a 10.45 ± 0.75 ab 7.44 ± 1.61 b 7.59 ± 1.09 b 9.72 ± 0.69 ab
Lignin 36.24 ± 0.71 b 56.17 ± 3.13 a 45.5 ± 5.07 ab 35.47 ± 1.45 b 32.94 ± 2.75 b 28.57 ± 1.26 a 34.54 ± 1.86 a 29.34 ± 2.83 a 35.62 ± 2.3 a 31.76 ± 4.2 a 26.8 ± 2.15 b 36.4 ± 2.72 a 34.19 ± 1.33 ab 33.55 ± 1.68 ab 26.88 ± 1.05 b
Lipid 24.65 ± 3.11 a 10.11 ± 1.93 b 16.35 ± 3.94 ab 22.37 ± 0.85 ab 24.32 ± 3.62 a 10.43 ± 0.35 b 23.73 ± 2.62 a 23.71 ± 2.24 a 19.37 ± 2.09 ab 17.52 ± 3.12 ab 23.96 ± 3.3 a 10.45 ± 1.02 b 15.64 ± 1.3 ab 15.05 ± 0.78 ab 23.02 ± 3.09 a
Protein 11.59 ± 1 a 11.91 ± 1.2 a 11.18 ± 0.5 a 14.69 ± 1.18 a 13.63 ± 1.63 a 14.6 ± 1.09 ab 15.61 ± 0.61 ab 18.52 ± 1.89 a 13.29 ± 1.26 b 13.4 ± 0.76 b 12.49 ± 1.39 a 14.27 ± 1.97 a 18.22 ± 2.84 a 19.2 ± 1.84 a 17.12 ± 1.06 a
Tannin 4.79 ± 0.45 a 5.92 ± 0.29 a 4.61 ± 0.52 a 4.57 ± 0.25 a 4.44 ± 0.94 a 7.43 ± 0.33 a 3.11 ± 0.47 b 2.58 ± 0.7 b 3.52 ± 0.41 b 4.06 ± 0.23 b 2.74 ± 0.26 a 3.33 ± 0.46 a 2.64 ± 0.26 a 2.94 ± 0.22 a 2.68 ± 0.15 a
UnsatHC 0.69 ± 0.07 a 0.84 ± 0.09 a 1 ± 0.12 a 0.93 ± 0.08 a 0.83 ± 0.06 a 1.53 ± 0.29 a 1.54 ± 0.24 a 1.57 ± 0.16 a 2.74 ± 0.41 a 2.05 ± 0.33 a 4 ± 0.29 a 3.61 ± 0.74 a 4 ± 0.33 a 4 ± 0.8 a 5 ± 0.73 a
Unnamed 2.08 ± 0.34 ab 1.12 ± 0.07 b 2.5 ± 0.42 a 1.8 ± 0.23 ab 1.91 ± 0.18 ab 3.92 ± 0.08 a 3.21 ± 0.33 a 3.77 ± 0.18 a 4.18 ± 0.1 a 4.47 ± 0.5 a 1.72 ± 0.12 bc 2.48 ± 0.14 a 1.33 ± 0.13 c 1.49 ± 0.2 bc 2.03 ± 0.2 ab

5.2 peaks

Class CPCRW baseline CPCRW drought CPCRW field moist CPCRW saturation CPCRW time zero saturation DWP baseline DWP drought DWP field moist DWP saturation DWP time zero saturation SR baseline SR drought SR field moist SR saturation SR time zero saturation
AminoSugar 1894 1513 1884 2236 2057 2661 1579 983 1621 2277 873 2016 2290 2278 1588
Carb 2138 1704 2249 2624 2499 3609 1784 1271 2198 3018 1348 2382 2871 2949 1874
ConHC 5087 6271 6303 5136 5145 4429 6301 3182 6658 6885 5186 7470 5489 5899 6485
Lignin 12092 13273 13956 12754 12219 11551 12105 6612 12358 13708 8067 14737 14305 14504 11664
Lipid 3244 3647 4531 4826 4248 3387 5272 2858 5515 5210 3042 4370 5540 5525 4852
Protein 5093 5028 5689 6193 5747 5274 6270 3450 5483 6107 3097 6081 6493 6813 5373
Tannin 2386 2325 2313 2317 2180 2560 1564 870 1955 2232 1334 2297 2031 2104 1934
total 34712 37243 40647 39526 37252 36393 38459 20940 39826 43499 25252 43463 43265 44388 37947
Unnamed 1340 1458 1609 1508 1362 1396 1438 720 1558 1692 840 1777 1521 1580 1665
UnsatHC 1438 2024 2113 1932 1795 1526 2146 994 2480 2370 1465 2333 2725 2736 2512

5.3 unique peaks

site Class drought field_moist saturation time_zero_sat
CPCRW AminoSugar 128 132 135 107
CPCRW Carb 127 162 171 149
CPCRW ConHC 581 592 397 399
CPCRW Lignin 856 875 733 655
CPCRW Lipid 459 580 598 520
CPCRW Protein 417 415 466 388
CPCRW Tannin 150 130 133 124
CPCRW Unnamed 181 202 190 175
CPCRW UnsatHC 252 246 255 228
CPCRW total 3151 3334 3078 2745
DWP AminoSugar 125 61 112 137
DWP Carb 107 62 148 157
DWP ConHC 500 208 619 579
DWP Lignin 819 279 872 901
DWP Lipid 642 241 711 577
DWP Protein 537 170 439 421
DWP Tannin 128 53 162 142
DWP Unnamed 194 73 215 212
DWP UnsatHC 275 110 315 280
DWP total 3327 1257 3593 3406
SR AminoSugar 175 157 150 110
SR Carb 185 167 150 105
SR ConHC 747 460 530 590
SR Lignin 965 846 860 617
SR Lipid 481 634 556 452
SR Protein 452 465 446 312
SR Tannin 216 130 146 149
SR Unnamed 222 182 178 212
SR UnsatHC 209 288 281 220
SR total 3652 3329 3297 2767